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KMID : 0360220230640121191
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Journal of the Korean Ophthalmological Society
2023 Volume.64 No. 12 p.1191 ~ p.1197
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Inflammatory Factors Predicting Dry Eye Syndrome in a Model Using Osmotic Pressure
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Park Keon-Woo
Koh Jae-Woong
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Abstract
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Purpose: To identify inflammatory factors that may serve as biomarkers for dry eye syndrome using a hyperosmotic dry eye syndrome model.
Methods: Cultured human conjunctival epithelial cells were subjected to 400, 450, 500, and 550 mOsm/L NaCl, and cell viability was assessed in response to osmolarity. The relative expression of inflammatory factors was evaluated by real-time polymerase chain reaction of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, metalloproteinase (MMP)-2, and MMP-9 according to the NaCl concentration.
Results: An increase in the concentration of NaCl led to a notable decrease in cell viability (p < 0.05). IL-8, TNF-α, and MMP-9 expression levels increased significantly in proportion to NaCl concentration (p < 0.05).
Conclusions: IL-8, TNF-α, and MMP-9 may serve as effective biomarkers in dry eye studies.
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KEYWORD
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Dry eye syndromes, Hyperosmolarity, Inflammatory factors
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